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Growth Assays to Assess Polyglutamine Toxicity in Yeast
JoVE Journal
Biology
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JoVE Journal Biology
Growth Assays to Assess Polyglutamine Toxicity in Yeast
DOI:

09:06 min

March 05, 2012

Chapters

  • 00:05Title
  • 01:25Experimental Considerations and Set-up of Yeast Cells Expressing Toxic PolyQ-expansion Proteins
  • 03:26Plating Assay
  • 04:30Spotting Assay
  • 05:25Liquid Growth Assay
  • 07:08Representative Growth Assay Results
  • 08:30Conclusion

Summary

Automatic Translation

This manuscript describes three complementary protocols for assessing the toxicity of polyglutamine (polyQ)-expansion proteins in the yeast Saccharomyces cerevisiae. These protocols can easily be modified to monitor the toxicity of other misfolded proteins in yeast.

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