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Chapters
- 00:05Title
- 01:49Preparing Human Articular Chondrocytes (HAC) for 3D Encapsulation
- 02:51Encapsulating HACs into 3D Beads
- 04:50Culturing Chondrocytes in Synovial Fluid
- 06:05Harvesting HACs in Alginate Beads for Gene Expression or Histological Analysis
- 07:363D Cultures
- 09:31Conclusion
A 3D system of culturing human articular chondrocytes in high levels of synovial fluid is described. Synovial fluid reflects the most natural microenvironment for articular cartilage, and can be easily obtained and stored. This system thus can be used for studying cartilage regeneration and for screening therapeutics for treating arthritis.
Tags
3D SystemCulturingHuman Articular ChondrocytesSynovial FluidCartilage DestructionOsteoarthritisDisabilityCartilage RegenerationChronic PainImmobilityScaffoldsBalanced MediumIn Vivo TransplantationChondrocyte GrowthMatrix DepositionNative Joint CavitySynovial FluidAvascular Articular CartilageGrowth FactorsCytokinesEnzymesChondrocyte MetabolismHyaluronanLubricinArtificial Media
