A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:48Setting up Organotypic Cerebellar Slice Cultures
- 03:02Fixation and Immunostaining of Organotypic Cerebellar Slice Cultures
- 04:52Measurement of Purkinje Cell Dendritic Tree Size and Branch Points
- 06:07Representative Results for the Purkinje Cell Dendritic Tree Size and Branching
- 07:23Conclusion
Tags
Purkinje CellDendritic MorphologyOrganotypic Slice CulturesSagittal PlanePostnatal PeriodAnti-Calbindin D28KEGFPJackson LabsCerebellar Slice CulturesDendritic DevelopmentDendritic ExpansionQuantitative EvaluationDendritic Tree SizeBranch Point NumbersAnti-calbindin StainedProtein Kinase C (PKC) Acti
