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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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Immunology and Infection
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JoVE Journal Immunology and Infection
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

DOI:
10.3791/3673-v

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07:56 min

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September 03, 2012

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, , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska-Lincoln, 2Department of Chemistry,University of Nebraska-Lincoln

Chapters

  • 00:05Title
  • 01:41Culturing M. tuberculosis (Class III Agent)
  • 04:44Adapting the NMR Methodology
  • 06:36Results: M. tuberculosis Wild Type Metabolic Pool
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

Tags

Mycobacterium TuberculosisNuclear Magnetic ResonanceMetabolomic StudiesSample PreparationDrug-resistant StrainsGene FunctionGene Expression AnalysisGlobal PatternsMetabolic CompositionSmall Molecular Weight ChemicalsTarget IdentificationAnti-tubercular ActivityNMR Technology

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