Journal
/
Immunology and Infection
/
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

DOI:
10.3791/3686-v

•

10:41 min

•

January 03, 2012

•

, , ,
1Department of Molecular Biology and Microbiology,University of Central Florida

Chapters

  • 00:05Title
  • 01:31Cell Intoxication and Permeabilization
  • 03:34Sample and Standard Toxin Preparation
  • 06:11Surface Plasmon Resonance
  • 07:08Representative Surface Plasmon Resonance Traces and Analysis
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

Tags

Toxin TranslocationSurface Plasmon ResonanceAB ToxinsCell-binding B SubunitVesicle CarriersEndoplasmic Reticulum (ER)Catalytic A ChainProtein-conducting ChannelCytosolic TargetToxin TraffickingLysosomesGolgi ApparatusSubcellular Fractionation ProtocolDigitonin PermeabilizationAnti-toxin A Chain AntibodyDetection Method

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3686?article_type=v

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
check_url/3686?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/3686?article_type=v

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
check_url/3686?article_type=v

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
check_url/3686?article_type=v

Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
check_url/3686?article_type=v

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
check_url/3686?article_type=v

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
check_url/3686?article_type=v

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
check_url/3686?article_type=v

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
check_url/3686?article_type=v

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Read Article