Name: July 2011: This Month in JoVE (Video) | JoVE Journal
Uploaded: 2011-07-01T09:00:00.000+00:00
Duration: 4 min 8 s
Description: Discover innovative neuroscience and biology research from JoVE's July 2011 issue. Explore visualized experiments that enhance reproducibility and deepen understanding of complex biological mechanisms.

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5 minutes

<h2><a href="https://www.jove.com/details.php?id=3054">Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes</a></h2>
H&#233;l&#232;ne Salmon1, 2, Ana Rivas-Caicedo1, 2, Fran&#231;ois Asperti-Boursin1, 2, Camille Lebugle1, 2, Pierre Bourdoncle1, 2, Emmanuel Donnadieu1, 2 1Institut Cochin, Universit&#233; Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France
This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.
<h2><a href="https://www.jove.com/details.php?id=2588">Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue</a></h2>
Graham Knott, St&#233;phanie Rosset, Marco Cantoni Centre of interdisciplinary electron microscopy, &#201;cole Polytechnique F&#233;d&#233;rale de Lausanne
This abstract describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focusssed ion beam, scanning electron microscope.
<h2><a href="https://www.jove.com/details.php?id=3103">High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation</a></h2>
David Filgueiras-Rama, Raphael Pedro Martins, Steven R. Ennis, Sergey Mironov, Jiang Jiang, Masatoshi Yamazaki, J&#233;r&#244;me Kalifa, Jos&#232; Jalife, Omer Berenfeld Center for Arrhythmia Research. Internal Medicine, University of Michigan 
This report provides a detailed description of the methodology and results of simultaneous endocardial and epicardial optical mapping of electrical excitation in the intact left atrium of a Langendorff-perfused sheep heart during stretch-induced atrial fibrillation.
<h2><a href="https://www.jove.com/details.php?id=2901">An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies</a></h2>
Nicholas Manoukis, Scott Geib, Danny Seo, Michael McKenney, Roger Vargas, Eric Jang Agricultural Research Service, US Dept. of Agriculture, US Pacific Basin Agricultural Research Center
Fopius arisanus is an egg-larval parasitoid of Tephritid fruit flies that is successfully used in biological control of these important tropical pests. We describe here an optimized protocol for rearing F. arisanus on a small scale using readily available materials.
<h2><a href="https://www.jove.com/details.php?id=2903">Shape Memory Polymers for Active Cell Culture</a></h2>
Kevin A. Davis, Xiaofan Luo, Patrick T. Mather, James H. Henderson Department of Biomedical and Chemical Engineering, Syracuse Biomaterials Institute
A method for developing cell culture substrates with the ability to change topography during culture is described. The method makes use of smart materials known as shape memory polymers that have the ability to memorize a permanent shape. This concept is adaptable to a wide range of materials and applications.
<h2><a href="https://www.jove.com/details.php?id=2965">Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis</a></h2>
Rei Kitani, Federico Kalinec Division of Cell Biology and Genetics, House Ear Institute
A reliable method to investigate outer hair cell (OHC) motile responses, including electromotility, slow motility and bending, is described. OHC motility is elicited by stimulation with an external alternating electrical field, and the method takes advantage of high-speed image recording, LED-based illumination, and last generation image analysis software.
<h2><a href="https://www.jove.com/details.php?id=2739">Measurement of Aggregate Cohesion by Tissue Surface Tensiometry</a></h2>
Christine M. Butler, Ramsey A. Foty Department of Surgery, UMDNJ-Robert Wood Johnson Medical School
We describe a method of measuring binding energy, expressible as tissue surface tension, between cells within 3D tissue-like aggregates. Differences in tissue surface tension have been demonstrated to correlate with invasiveness of lung, muscle, and brain tumors, and are fundamental determinants of establishing spatial relationships between different cell types.
<h2><a href="https://www.jove.com/details.php?id=2502">Quantitative Autonomic Testing</a></h2>
Peter Novak Department of Neurology, University of Massachusetts Medical School
Standardized, comprehensive and fully quantitative testing of autonomic functions is described. The autonomic tests consist of evaluation of all three major autonomic domains including cardiovagal, adrenergic and sudomotor. The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores.
<h2><a href="https://www.jove.com/details.php?id=2920">Morris Water Maze Test for Learning and Memory Deficits in Alzheimer&#39;s Disease Model Mice</a></h2>
Kelley Bromley-Brits*, Yu Deng*, Weihong Song Department of Psychiatry, Brain Research Center, University of British Columbia * These authors contributed equally
The Morris Water Maze is a behavioral task to test hippocampal-dependent learning and memory. It has been widely used in the study of neurobiology, neuropharmacology and neurocognitive disorders in rodent models.

july 2011: this month in jove

July 2011: This Month in JoVE

Here are some highlights from the July 2011 Issue of Journal of Visualized Experiments (JoVE).

Research

JoVE Journal

Biology

Discover innovative neuroscience and biology research from JoVE's July 2011 issue. Explore visualized experiments that enhance reproducibility and deepen understanding of complex biological mechanisms.

July 2011: This Month in JoVE (Video) | JoVE Journal

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July 2011: This Month in JoVE

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