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JoVE Journal Bioengineering
Site-specific Ingegneria batterica Cromosoma
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Site-specific Ingegneria batterica Cromosoma: ΦC31 integrasi Mediated Cassette Exchange (IMCE)

Article DOI: 10.3791/3698-v • 08:21 min • March 16th, 2012
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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Un metodo rapido ed efficace per integrare DNA estraneo di interesse in pre-fatti ceppi accettori, chiamati ceppi pista di atterraggio, è descritta. Il metodo permette integrazione sito-specifica di una cassetta DNA nel locus ingegnerizzato atterraggio di un dato ceppo, tramite coniugazione e l'espressione del ΦC31 integrasi.

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Bioingegneria Numero 61 ΦC31 integrasi Rhizobiales Ingegneria cromosomica genetica batterica
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