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Chapters
- 00:05Title
- 01:45Preparation of Cell Suspension from Bone Marrow
- 03:49Phenotypic Analysis
- 06:00Magnetic Enrichment of c-Kit+ Cells
- 08:11Representative Results
- 11:24Conclusion
A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.
Tags
Phenotypic AnalysisIsolationMurine Hematopoietic Stem CellsLineage-committed ProgenitorsBone MarrowHSCsBlood Cell LineagesMolecular DissectionHomeostasisHematopoiesisOncologyRegenerative MedicineLabeling ProtocolFluorescent AntibodiesElectronic Gating ProcedureFlow CytometryHematopoietic Progenitor SubsetsDistributionIndividual MiceMagnetic EnrichmentC-Kit ExpressionTrabecular OsteoblastsSinusoidal EndotheliumFunctional NichesN-cadherin+ OsteoblastsReceptor Tyrosine Kinase Tie2Angiopoietin-1
