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JoVE Journal
Immunology and Infection

This content is Open Access.

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
 

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Article DOI: 10.3791/3757-v 11:46 min March 8th, 2012
March 8th, 2012

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Summary

This article describes a method to visualize formation of an HIV-1 envelope-induced virological synapse on glass supported planar bilayers by total internal reflection fluorescence (TIRF) microscopy. The method can also be combined with immunofluorescence staining to detect activation and redistribution of signaling molecules that occur during HIV-1 envelope-induced virological synapse formation.

Tags

HIV-1 Envelope Virological Synapse Signaling Synthetic Lipid Bilayers Cell To Cell Transmission CD4+ T Cells F-actin Gp120 Chemokine Receptor (CKR) CCR5 CXCR4 LFA-1 ICAM Family Virus-infected Donor Cells Target Cells HIV-1 Virions Intracellular Signaling Events TCR Signalosome Lck CD3ζ ZAP70 LAT SLP-76 Itk PLCγ Supramolecular Arrangement
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