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April 30, 2012
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A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.
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Mammary Epithelial CellsThree-dimensional Mixed-cell Spheroid Co-cultureSignaling PathwaysMoleculesNormal Cell BehaviorMalignant Cell BehaviorTwo-dimensional Cell Culture ModelsIntracellular Signaling PathwaysExtracellular ForcesDimensionalityCell Surface TensionThree-dimensional ModelsMicroenvironmentTumorsTumorigenesisTumor BehaviorTumor StromaBidirectional SignalsStromal CellsFibroblasts
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Xu, K., Buchsbaum, R. J. Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture. J. Vis. Exp. (62), e3760, doi:10.3791/3760 (2012).
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