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Biology

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Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
 

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

Article DOI: 10.3791/3760
April 30th, 2012

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Summary April 30th, 2012

A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.

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