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Chapters
- 00:05Title
- 01:08Establishing Three-dimensional Co-cultures
- 02:54Isolation of Cells from Spheroid Co-cultures
- 04:22Representative Imaging and Analysis of the Effects of Fibroblasts on Epithelial Cells Grown in 3D Spheroid Co-cultures
- 07:05Conclusion
A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.
Tags
Mammary Epithelial CellsThree-dimensional Mixed-cell Spheroid Co-cultureSignaling PathwaysMoleculesNormal Cell BehaviorMalignant Cell BehaviorTwo-dimensional Cell Culture ModelsIntracellular Signaling PathwaysExtracellular ForcesDimensionalityCell Surface TensionThree-dimensional ModelsMicroenvironmentTumorsTumorigenesisTumor BehaviorTumor StromaBidirectional SignalsStromal CellsFibroblasts
