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JoVE Journal Biology
Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
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Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

Article DOI: 10.3791/3760
April 30th, 2012 •
1, 1
1Molecular Oncology Research Institute, Department of Medicine, Tufts Medical Center

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April 30th, 2012

A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.

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