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Chapters
- 00:05Title
- 01:45Maintaining Flies and Collecting Eggs
- 03:43Preparing Coverslips and Slides
- 05:10Dechorionating Embryos
- 06:36Provoking the SAC by Microinjecting Embryos with Appropriate Reagents
- 08:55Imaging Embryos
- 10:08Results: Observing Dynamic Kinetochore Behavior and Chromosome Motion
- 13:28Conclusion
The kinetochore is where the SAC initiates its signal monitoring the mitotic segregation of the sister chromatids. A method is described to visualize the recruitment and turnover of one of the kinetochore proteins and its coordination with the chromosome motion in Drosophila embryos using a Leica laser scanning confocal system.
Tags
Mitotic CheckpointKinetochore ProteinChromosome MotionLiving Drosophila Syncytial EmbryosSAC MechanismAneuploidyGenomic InstabilityCancersBirth DefectsAlzheimer’sMad1Mad2Bub1BubR1Bub3Mps1Zw10RodAurora B KinaseCdc20APC/C ActivatorsCyclin BSecurinMetaphase To Anaphase TransitionSAC Signal InitiationKinetochore AssemblyRelay Onto APC/C InhibitionDrosophila Experimental System
