Name: August 2011: This Month in JoVE (Video) | JoVE Journal
Uploaded: 2011-08-01T11:00:00.000+00:00
Duration: 4 min 37 s
Description: Discover key neuroscience and biology highlights from JoVE's August 2011 issue. Explore innovative visualized experimental techniques and research trends advancing scientific methodology.

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5 minutes

<h2><a href="https://www.jove.com/details.php?id=2715">Examining the Characteristics of Episodic Memory using Event-related Potentials in Patients with Alzheimer's Disease</a></h2>
Erin Hussey, Brandon Ally Department of Neurology, Vanderbilt University
The methodology for collecting high-density event-related potential data while patients with Alzheimer's disease perform a recognition memory task is reviewed. This protocol will include subject preparation, quality assurance, data acquisition, and data analysis.
<h2><a href="https://www.jove.com/details.php?id=2753">Axon Stretch Growth: The Mechanotransduction of Neuronal Growth</a></h2>
Joseph R. Loverde1, 2, Rosa E. Tolentino1, 2, Bryan J. Pfister1 1Departments of Biomedical Engineering, New Jersey Institute of Technology, 2Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey
A unique tissue engineering method was developed to elongate numerous nerve fibers in culture by recapitulating axon stretch growth; a form of nervous system growth whereby nerves elongate in conjunction with growth of the enlarging body.
<h2><a href="https://www.jove.com/details.php?id=2829">Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field</a></h2>
John Y. Takekawa1, Nichola J. Hill1, 2, Annie K. Schultz1, Samuel A. Iverson1, Carol J. Cardona3, 4, Walter M. Boyce2, Joseph P. Dudley5 1USGS Western Ecological Research Center, 2Wildlife Health Center, University of California, Davis, 3Department of Population Health and Reproduction, University of California, Davis, 4Department of Veterinary and Biomedical Sciences, University of Minnesota , 5Science Applications International Corporation
This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid diagnosis.
<h2><a href="https://www.jove.com/details.php?id=2832">Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved</a></h2>
Robert H.S. Kraus1, Pim van Hooft1, Jonas Waldenstr&#246;m2, Neus Latorre-Margalef2, Ronald C. Ydenberg1, 3, Herbert H.T. Prins1 1Resource Ecology Group, Wageningen University, 2Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences, Linnaeus University, 3Centre for Wildlife Ecology, Simon Fraser University 
A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.
<h2><a href="https://www.jove.com/details.php?id=2852">Time-lapse Microscopy Of Early Embryogenesis in Caenorhabditis elegans</a></h2>
Lynn Boyd1, Connie Hajjar1, Kevin O'Connell2 1Department of Biological Sciences, University of Alabama in Huntsville, 2NIDDK-National Institutes of Health
This article describes a technique for the visualization of the early events of embryogenesis in the nematode Caenorhabditis elegans.
<h2><a href="https://www.jove.com/details.php?id=2938">Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton</a></h2>
Xiquan Gao1, Robert C. Britt Jr.1, Libo Shan2, Ping He1 1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&#38;M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&#38;M University
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
<h2><a href="https://www.jove.com/details.php?id=2949">Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures</a></h2>
Dietmar Plenz, Craig V. Stewart, Woodrow Shew, Hongdian Yang, Andreas Klaus, Tim Bellay Section on Critical Brain Dynamics, National Institute of Mental Health
A robust way to study neuronal avalanches, i.e. scale-invariant spatio-temporal activity bursts, indicative of critical state dynamics in cortex. Avalanches emerge spontaneously in developing superficial layers of cultured cortex which allows for long-term measurements of the activity with planar integrated multi-electrode arrays (MEA) under precisely controlled conditions.
<h2><a href="https://www.jove.com/details.php?id=3007">Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy</a></h2>
Ian Sharp1, James Patton1, Molly Listenberger2, Emily Case2 1Department of Bioengineering, University of Illinois at Chicago and Rehabilitation Institute of Chicago, 2Sensory Motor Performance Program, Rehabilitation Institute of Chicago
Recently, a vast amount of prospects have come available for human-robot interactive systems. In this paper we outline the integration of a new robotic device with open source software that can rapidly make possible a library of interactive functionality. We then outline a clinical application for a neurorehabilitation application.
<h2><a href="https://www.jove.com/details.php?id=3058">Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica</a></h2>
John D. Chan, Jonathan S. Marchant Department of Pharmacology and The Stem Cell Institute, University of Minnesota Medical School
An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

august 2011: this month in jove

August 2011: This Month in JoVE

Here are some highlights from the August 2011 Issue of Journal of Visualized Experiments (JoVE).

Research

JoVE Journal

Biology

Discover key neuroscience and biology highlights from JoVE's August 2011 issue. Explore innovative visualized experimental techniques and research trends advancing scientific methodology.

August 2011: This Month in JoVE (Video) | JoVE Journal

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August 2011: This Month in JoVE

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