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JoVE Journal
Immunology and Infection

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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
 

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

Article DOI: 10.3791/3807
February 8th, 2012

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Summary February 8th, 2012

Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.

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