/
/
Uno screening genetico per isolare Toxoplasma gondii Host-cell Egress Mutants
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:39Titration of Mutagen
- 05:59Enrichment of Egress Mutants
- 08:48Validation of Mutant Phenotypes by Egress Assays
- 10:21Cloning of Egress Mutants by Limiting Dilution
- 12:41Representative Data
- 14:14Conclusion
Forward genetica è un metodo efficace per svelare il livello molecolare di come<em> Toxoplasma</em> Egresses dalla sua cellula ospite. I protocolli sono fornite mutagenizzare chimicamente parassiti, per arricchire mutanti con difetti in uscita indotta, e convalidare il fenotipo di mutanti clonati.
Tags
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
