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Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
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Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

Article DOI: 10.3791/3813 • 09:42 min • June 9th, 2012
June 9th, 2012 •
1, 1, 1, 1
1Nemours Biomedical Research, Alfred I. duPont Hospital for Children

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We developed a new protocol to improve efficiency of in vitro differentiation of mouse embryonic stem cells into motor neurons. The differentiated ES cells acquired motor neurons features as evidenced by expression of neuronal and motor neuron markers using immunohistochemical techniques.

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Mouse Embryonic Stem Cells Motor Neurons Differentiation Retinoic Acid Sonic Hedgehog Disease Mechanisms Drug Compounds Spinal Muscular Atrophy Amyotrophic Lateral Sclerosis Protocols Neuralization Process Noggin Fibroblast Growth Factors FGF Signaling Neurogenesis Anterior Neural Patterning Posterior Neural Identity
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