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JoVE Journal
Immunology and Infection
Author Produced

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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
 

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Article DOI: 10.3791/3822
September 9th, 2012

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Summary September 9th, 2012

This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.

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