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Immunology and Infection
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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

DOI:
10.3791/3822-v

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13:08 min

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September 09, 2012

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, ,
1Applied Bioscience Program, Faculty of Science,University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences,University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences,University of Ontario Institute of Technology

Chapters

  • 00:05Title
  • 03:07FV 1-Stage Microplate Activity Assay
  • 09:27FV 2-Stage Microplate Activity Assay
  • 11:31FV Activity in Normal Human Plasma and in Patients With Disseminated Intravascular Coagulation (DIC)
  • 12:15Conclusion

Summary

Automatic Translation

Automatic Translation

This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.

Tags

Factor V ActivityHuman PlasmaMicroplate Coagulation AssayBlood CoagulationThrombinFibrinogenClot FormationProthrombinaseFV AssaysAutomated FV AssaysMicroplate PlatformEnzyme-catalyzed EventsClot LysisPlatelet AggregationCoagulation FactorsFV Activity MeasurementKinetic Microplate ReaderAbsorbance Change At 405nm

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