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A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Article DOI: 10.3791/3850
April 24th, 2012 •
1, 1, 1
1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

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April 24th, 2012

A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

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