Blood Collection for Biochemical Analysis in Adult Zebrafish
May 26th, 2012
This paper presents a technique for collection of blood from the dorsal aorta of Zebrafish. It also provides instructions for obtaining serum for use in biochemical analyses, such as tests for determining cholesterol and triglyceride levels.
In this Video, we aim to show a technique of blood collection in zebrafish. This technique needs a container for anesthesia, a Petri dish, and a scalpel, low rotation tips and automatic micropipet, P 20 and micro tubes, and the centrifuge. Before I Start the blood collection, it's necessary to anesthetize the fish with a fishing net.
The fish is moved from the aquarium to a container that has a aquarium water and ice chips. The time of Anesthesia depends on each fish. It usually takes from three to six Seconds.
The fish is considered anesthetized. When it does not respond to a sternal stimulus, the fish has to be gently transferred from anesthesia container to a dry gauze to remove the excess of water. When the fish Is anesthetized and the excess of water is removed, the fish is gently involved in a dry gauze that covers body and head, leaving free access to the tail.
With a scalpel, a transverse incision is done just before the tail and the blood starts to Flow. The Collected Blood is then replaced into a micro tube. The tube should be gently handled to avoid hemolysis.
Once the blood is collected, it's necessary to centrifuge to obtain the serum. It is possible to use a pool of blood samples since the time between the first blood collection and the centrifuge do not exceed 10 minutes. Moreover, the blood should be centrifuged for 10 minutes.
These steps should be rigorously followed in order to avoid demo moly of the samples I.
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Overview
This paper presents a technique for the collection of blood from the dorsal aorta of zebrafish. The method includes instructions for obtaining serum for biochemical analyses, such as cholesterol and triglyceride level tests.
Key Study Components
Area of Science
- Neuroscience
- Biochemistry
- Zebrafish Research
Background
- Blood collection techniques are essential for various biochemical analyses.
- Zebrafish serve as a model organism in biological research.
- Understanding serum composition can aid in metabolic studies.
- Cholesterol and triglyceride levels are important indicators of health.
Purpose of Study
- To demonstrate a reliable method for blood collection in zebrafish.
- To provide a protocol for serum extraction for biochemical tests.
- To facilitate research on metabolic processes in zebrafish.
Methods Used
- Use of a container for anesthesia with aquarium water and ice chips.
- Transfer of zebrafish using a fishing net.
- Application of a scalpel for blood collection from the dorsal aorta.
- Utilization of centrifuge for serum separation.
Main Results
- Successful blood collection technique demonstrated.
- Instructions for serum extraction provided.
- Method validated for biochemical analysis of cholesterol and triglycerides.
- Technique adaptable for various research applications.
Conclusions
- The technique is effective for blood collection in zebrafish.
- Provides a foundation for further biochemical studies.
- Enhances the understanding of metabolic processes in aquatic models.
