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Blood Collection for Biochemical Analysis in Adult Zebrafish
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Blood Collection for Biochemical Analysis in Adult Zebrafish

Blood Collection for Biochemical Analysis in Adult Zebrafish

DOI:
10.3791/3865-v

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03:11 min

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May 26, 2012

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, , , , ,
1Hospital de Clínicas de Porto Alegre,Centro de Pesquisa Experimental Laboratório de Hepatologia e Gastroenterologia Experimental, 2Universidade Federal do Rio Grande do Sul, UFRGS. Porto Alegre, RS, Brasil

Chapters

  • 00:02Title
  • 00:39Removal from the Aquarium to Anesthesia
  • 00:59Anesthesia
  • 01:15After Anesthesia
  • 01:39Tail Cut
  • 02:07Collected Blood
  • 02:23The Centrifuge

Summary

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Automatic Translation

This paper presents a technique for collection of blood from the dorsal aorta of Zebrafish. It also provides instructions for obtaining serum for use in biochemical analyses, such as tests for determining cholesterol and triglyceride levels.

Tags

Blood CollectionBiochemical AnalysisAdult ZebrafishAnimal ModelHuman DiseasesPreclinical PlatformMolecular EventsTherapeutic StrategiesPhysiological MechanismsZebrafish PhysiologyOrgans And SystemsBlood Collection TechniqueLateral IncisionDorsal AortaHigh-precision TechniqueSkilled PractitionerWhole Blood Glucose LevelsDecapitations With ScissorsHeparinized Microcapillary Collection TubeHemolysisAppropriate Storage Temperature

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