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Biology

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Isolation and Primary Culture of Rat Hepatic Cells
 

Isolation and Primary Culture of Rat Hepatic Cells

Article DOI: 10.3791/3917
June 29th, 2012

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Summary June 29th, 2012

Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.

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