Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Isolation and Primary Culture of Rat Hepatic Cells

Isolation and Primary Culture of Rat Hepatic Cells

Article DOI: 10.3791/3917
June 29th, 2012


Summary June 29th, 2012

Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter