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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
 

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

Article DOI: 10.3791/3951
October 28th, 2012

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Summary October 28th, 2012

Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.

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