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Planarian Immobilization, Partial Irradiation, and Tissue Transplantation

Article DOI: 10.3791/4015 • 10:09 min • August 6th, 2012
August 6th, 2012 •
1,2, 3,4
1Department of Neurobiology and Anatomy, University of Utah School of Medicine, 2Department of Molecular, Cellular and Developmental Biology, UCSB, 3Howard Hughes Medical Institute, 4Stowers Institute for Medical Research

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Summary
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An effective method for grafting tissue of defined and consistent size between planaria is described. Also included is a description of how the immobilization technique used for transplantation can be adapted, in conjunction with lead shields, for the partial irradiation of live animals.

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Planarian Immobilization Partial Irradiation Tissue Transplantation Metazoan Regeneration Stem Cell Biology Neoblasts Pluripotent Stem Cells Cellular Behaviors Subpopulations Of Stem Cells Molecular Study Functional Study Tissue Transplantation Advantages Partial Irradiation Advantages Cell Autonomous Factors Non-autonomous Factors
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