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Preparing T Cell Growth Factor from Rat Splenocytes
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Preparing T Cell Growth Factor from Rat Splenocytes

Article DOI: 10.3791/402 • 18:27 min • October 31st, 2007
October 31st, 2007 •
1, 1
1Department of Physiology and Biophysics, University of California, Irvine (UCI)

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Summary
Automatic Translation

We describe the preparation of T cell growth factor used for the in vitro expansion of antigen-specific rat T lymphocyte lines.

Tags

T Cell Growth Factor Rat Splenocytes Antigen-specific T Cell Lines Interleukin 2 Th1 Polarization TCGF Preparation Cytokines Large-scale Production Rat Splenocyte Culture Supernatants Concanavalin A Mitogen Cytokine Production Sterile Filtration Storage At -20°C
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