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Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
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Bioengineering
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JoVE Journal Bioengineering
Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

DOI:
10.3791/4024-v

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08:51 min

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May 08, 2012

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, ,
1School of Biochemistry,University of Bristol, 2Smith and Nephew

Chapters

  • 00:05Title
  • 01:34Coating Surfaces with Matrix Ligand
  • 02:54Preparation of Cells
  • 03:50Preparing the Low-intensity Pulsed Ultrasound (LIPUS) Stimulation
  • 05:10Focal Adhesion Analysis by Immunofluorescence
  • 05:58Biochemical Assays
  • 06:24Representative Results
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.

Tags

Low-intensity UltrasoundAdhesion-dependent SignalsMulticellular OrganismsCell BehaviorExtracellular MatrixMatrix-engagementCell MigrationCell ProliferationCell SecretionCell DifferentiationMolecular InteractionsExtracellular Matrix ReceptorsIntegrinsMechanical LinkAdhesion-dependent Signaling CascadesTransmembrane ReceptorsSyndecan-4α5β1-integrinFibronectinSyndecan-4 SignalingFibroblast MigrationHealing RetardationFibronectin-dependent SignalingRepair Processes

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