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July 06, 2012
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Summary
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A technique to identify translational pause sites on mRNA is described. This procedure is based on isolation of nascent polypeptides accumulating on ribosomes during in vitro translation of a target mRNA, followed by the size analysis of the nascent chains using a denaturing gel electrophoresis.
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Cite this Article
Jha, S. S., Komar, A. A. Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA. J. Vis. Exp. (65), e4026, doi:10.3791/4026 (2012).
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