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07:09 min
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July 31, 2012
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Summary
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In the post-human genomics era, the availability of recombinant proteins in native conformations is crucial to structural, functional and therapeutic research and development. Here, we describe a test- and large-scale protein expression system in human embryonic kidney 293T cells that can be used to produce a variety of recombinant proteins.
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Cite this Article
Aydin, H., Azimi, F. C., Cook, J. D., Lee, J. E. A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells. J. Vis. Exp. (65), e4041, doi:10.3791/4041 (2012).
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