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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
JoVE Journal
Biology
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JoVE Journal Biology
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

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07:09 min

July 31, 2012

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07:09 min
July 31, 2012

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Summary

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In the post-human genomics era, the availability of recombinant proteins in native conformations is crucial to structural, functional and therapeutic research and development. Here, we describe a test- and large-scale protein expression system in human embryonic kidney 293T cells that can be used to produce a variety of recombinant proteins.

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