Analysis of Single-cell Gene Transcription by RNA Fluorescent <em>In Situ</em> Hybridization (FISH)

Elena Ronander; Dominique C. Bengtsson; Louise Joergensen; Anja T. R. Jensen; David E. Arnot

doi:10.3791/4073

Journal

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Biology

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Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

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Biology
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Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

15,360 Views

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13:06 min

•

October 07, 2012

DOI:

10.3791/4073-v

DOI:

10.3791/4073-v

•

13:06 min

October 07, 2012

•

1 Views

Elena Ronander^1,2, Dominique C. Bengtsson^1,2, Louise Joergensen^1,2, Anja T. R. Jensen^1,2, David E. Arnot^1,2,3

¹Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences,University of Copenhagen, ²Department of Infectious Diseases,Copenhagen University Hospital (Rigshospitalet), ³Institute of Infection and Immunology Research, School of Biology,University of Edinburgh

Summary

Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes ¹. The technique is adaptable and can be used on different types of genes, cells and organisms.