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1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences,University of Copenhagen, 2Department of Infectious Diseases,Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology,University of Edinburgh
Chapters
- 00:00Title
- 02:00Day 1
- 02:43Day 2
- 02:58Reagents and Materials
- 04:14Thin Smears and Fixation of Parasites Prior to in situ Hybridization
- 07:06Hybridization of RNA Probes to Fixed Slides
- 08:51Antibody Conjugation & Fluorescence Amplification
- 11:12Visualization of Hybridized Probes
- 11:27Representative Results & Discussion
Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes 1. The technique is adaptable and can be used on different types of genes, cells and organisms.
Tags
Single-cell Gene TranscriptionRNA Fluorescent In Situ Hybridization (FISH)Plasmodium Falciparum Infected ErythrocytesPfEMP1 Protein VariantsVar GenesMutually Exclusive RegulationTranscription AssaysRNA-FISH MethodologyDigoxigenin-labeled Antisense RNA ProbesBiotin-labeled Antisense RNA ProbesTSA Plus Fluorescence Palette System
