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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
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Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Article DOI: 10.3791/4151
June 22nd, 2012 •
*1,2, *1,2, 1,2
1Department of Chemistry & Biochemistry, University of Colorado Boulder, 2Biofrontiers Institute, University of Colorado Boulder
* These authors contributed equally

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Summary
Automatic Translation
June 22nd, 2012

This protocol describes an extrusion method for preparing lipid vesicles of sub-micron sizes with a high degree of homogeneity. This method uses a pressure-controlled system with controlled nitrogen flow rates for liposome preparation. The lipid preparation1,2, liposome extrusion, and size characterization will be presented herein.

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