/
/
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:13Liposome Preparation
- 02:46Extrusion
- 05:28Dynamic Light Scattering (DLS) Analysis
- 06:18Nanoparticle Tracking Analysis (NTA)
- 07:35Results: Analysis of Nano-sized Lipid Vesicles
- 11:06Conclusion
This protocol describes an extrusion method for preparing lipid vesicles of sub-micron sizes with a high degree of homogeneity. This method uses a pressure-controlled system with controlled nitrogen flow rates for liposome preparation. The lipid preparation1,2, liposome extrusion, and size characterization will be presented herein.
Tags
Pressure-controlled Extrusion MethodNano-sized Lipid VesiclesLiposomesPhospholipidsProtein-protein InteractionsProtein-lipid InteractionsDrug DeliveryEncapsulationCurved Lipid BilayersLarge Unilamellar Vesicles (LUVs)Small Unilamellar Vesicles (SUVs)Multilamellar Vesicles (MLVs)Membrane CurvatureCell SignalingEndo- And ExocytosisMembrane FusionProtein TraffickingLiposome-drug EncapsulationDrug EncapsulationExtrusion Step
