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Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
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Chapters
- 00:05Title
- 01:38Assay Set-up
- 03:41Cell Staining and Counting
- 05:56Results: Measurements of the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
- 07:01Conclusion
Interstitial fluid flow is elevated in solid tumors and can modulate tumor cell invasion. Here we describe a technique to apply interstitial fluid flow to cells embedded in a matrix and then measure its effects on cell invasion. This technique can be easily adapted to study other systems.
Tags
Three-dimensional Cell CultureInterstitial Fluid FlowTumor Cell InvasionTumor MicroenvironmentStroma-associated SignalingBiophysical ForcesMatrix DensityStiffnessStructureInterstitial Fluid PressureInterstitial Fluid Flow VelocityTumor-induced AngiogenesisVascular PermeabilityInvasion Of Cancer CellsAutologous Chemotactic GradientsMatrix Metalloproteinase (MMP) Expression
