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Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
DOI:

12:53 min

January 08, 2013

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Chapters

  • 00:05Title
  • 01:49Preparation of Sample
  • 02:30Start-up of the SWAXS Machine: Source Cold Start-up Procedure
  • 03:25Start-up of the SWAXS Machine: Chiller Start-up and Shutdown Procedure
  • 04:36Turning on the Vacuum and Detector System Set-up
  • 05:48Calibration of Detectors
  • 07:38Software Procedure
  • 08:34Source Shutdown Procedure
  • 09:15Results: SWAXS Analysis of Macromolecules
  • 12:10Conclusion

Summary

Automatic Translation

The demonstration of the small and wide angle X-ray scattering (SWAXS) procedure has become instrumental in the study of biological macromolecules. Through the use of the instrumentation and procedures of specific angle methods and preparation, the experimental data from the SWAXS displays the atomic and nano-scale characterization of macromolecules.

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