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JoVE Journal
Biology

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Electricity-Free, Sequential Nucleic Acid and Protein Isolation
 

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Article DOI: 10.3791/4202
May 15th, 2012

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Summary May 15th, 2012

A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays.

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