Journal
/
Medicine
/
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

DOI:
10.3791/4205-v

•

10:12 min

•

July 10, 2012

•

,
1Strategic Research Center for Stem Cell Biology and Cell Therapy,University of Lund, 2Department of Cardiology Lund University Hospital,University of Lund

Chapters

  • 00:05Title
  • 02:04Isolation of the Cardiac Nuclei
  • 04:43Immunostaining for Flow Cytometry
  • 05:55Flow Cytometry
  • 07:51Results: Cardiomyocyte Nuclei Isolation by PCM-1 Positivity
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.

Tags

IsolationCardiomyocyte NucleiDensity SedimentationImmunolabelingAntibodiesPericentriolar Material 1 (PCM-1)Flow Cytometry SortingHigh Throughput AnalysisFrozen Archival MaterialBiobanksCarbon-14 DatingCell-cycle AnalysisThymidine AnaloguesBrdUIdUTranscriptome AnalysisEpigenetic Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4205?article_type=v

Isolation of Human Islets from Partially Pancreatectomized Patients
check_url/4205?article_type=v

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
check_url/4205?article_type=v

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
check_url/4205?article_type=v

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
check_url/4205?article_type=v

Isolation of Atrial Myocytes from Adult Mice
check_url/4205?article_type=v

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
check_url/4205?article_type=v

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
check_url/4205?article_type=v

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
check_url/4205?article_type=v

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice
check_url/4205?article_type=v

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Read Article