16,924 Views
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10:12 min
July 10, 2012
DOI:
10.3791/4205-v
Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.
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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cite this Article
Bergmann, O., Jovinge, S. Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue. J. Vis. Exp. (65), e4205, doi:10.3791/4205 (2012).
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