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Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

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10:12 min

July 10, 2012

DOI:

10:12 min
July 10, 2012

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Summary

Automatically generated

Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.

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