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JoVE Journal
Immunology and Infection

This content is Open Access.

Mass Spectrometric Analysis of Glycosphingolipid Antigens
 

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Article doi: 10.3791/4224
April 16th, 2013

Summary April 16th, 2013

A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.

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