This protocol describes a method for visualizing a DNA double-strand break signaling protein activated in response to DNA damage as well as its localization during mitosis.
Beckta, J. M., Henderson, S. C., Valerie, K. Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins. J. Vis. Exp. (67), e4251, doi:10.3791/4251 (2012).