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Chapters
- 00:05Title
- 01:23Protein Expression and Purification
- 03:41DNA Purification
- 07:35Protein-DNA Complex Formation and Analysis
- 08:35Results: Crystal Structure of SeqA delta(41-59)-A25R Bound to Hemimethylated DNA
- 11:08Conclusion
Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.
Tags
DNA DuplexesCrystallizationSeqA-DNA ComplexesEscherichia Coli SeqANegative RegulatorDNA ReplicationPremature Reinitiation EventsHemimethylated GATC ClustersOrigin Of ReplicationReplication ForksHigher Ordered StructuresHigh Affinity ComplexesMinimal Functional UnitDimerGATC SequencesMolecular LevelStructural AnalysisProtein-DNA CrystallizationPacking InteractionsProtein-protein Interactions
