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Biology

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Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
 

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Article DOI: 10.3791/4266
November 1st, 2012

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Summary November 1st, 2012

Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.

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