Advances in mass spectrometry have allowed the high throughput analysis of protein expression and modification in a host of tissues. Combined with subcellular fractionation and disease models, quantitative mass spectrometry and bioinformatics can reveal new properties in biological systems. The method described herein analyzes chromatin-associated proteins in the setting of heart disease and is readily applicable to other in vivo models of human disease.
Monte, E., Chen, H., Kolmakova, M., Parvatiyar, M., Vondriska, T. M., Franklin, S. Quantitative Analysis of Chromatin Proteomes in Disease. J. Vis. Exp. (70), e4294, doi:10.3791/4294 (2012).