Quantitative Analysis of Chromatin Proteomes in Disease

Emma Monte; Haodong Chen; Maria Kolmakova; Michelle Parvatiyar; Thomas M. Vondriska; Sarah Franklin

doi:10.3791/4294

Journal

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Medicine

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Quantitative Analysis of Chromatin Proteomes in Disease

JoVE Journal
Medicine

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JoVE Journal Medicine

Quantitative Analysis of Chromatin Proteomes in Disease

13,015 Views

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08:11 min

•

December 28, 2012

DOI:

10.3791/4294-v

DOI:

10.3791/4294-v

•

08:11 min

December 28, 2012

•

4 Views

Emma Monte¹, Haodong Chen¹, Maria Kolmakova¹, Michelle Parvatiyar¹, Thomas M. Vondriska^1,2,3, Sarah Franklin^1,4

¹Department of Anesthesiology,David Geffen School of Medicine at UCLA, ²Department of Medicine,David Geffen School of Medicine at UCLA, ³Department of Physiology,David Geffen School of Medicine at UCLA, ⁴Department of Internal Medicine,Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah

Summary

Advances in mass spectrometry have allowed the high throughput analysis of protein expression and modification in a host of tissues. Combined with subcellular fractionation and disease models, quantitative mass spectrometry and bioinformatics can reveal new properties in biological systems. The method described herein analyzes chromatin-associated proteins in the setting of heart disease and is readily applicable to other in vivo models of human disease.

Quantitative Analysis of Chromatin Proteomes in Disease

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