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JoVE Journal Chemistry
Fastställande av gasfas Acidities av Oligopeptider
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Fastställande av gasfas Acidities av Oligopeptider

Article DOI: 10.3791/4348-v • 11:00 min • June 24th, 2013
June 24th, 2013 •
1, 1, 1, 1, 1
1Department of Chemistry, University of the Pacific

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Fastställandet av gas-fas acidities av cysteininnehållande oligopeptider beskrivs. Experimenten utförs med användning av en trippel kvadrupol masspektrometer. De relativa acidities av peptiderna mäts med kollisionen-dissociation experiment, och de kvantitativa acidities fastställs med utökade Cooks kinetisk metod.

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Chemistry Biochemistry Molecular Biology surhetsgrad kinetisk metod sammanstötning-dissociation trippel-kvadrupol masspektrometri oligopeptider peptider masspektrometri MS
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