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High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
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Bioengineering
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JoVE Journal Bioengineering
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

DOI:
10.3791/4415-v

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09:14 min

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September 13, 2012

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,
1School of Biological and Health Systems Engineering,Arizona State University

Chapters

  • 00:05Title
  • 01:26MEA Preparation and Cell Seeding
  • 03:58Site-specific Transfection of siRNA and Electroporation
  • 08:00Results: High Efficiency, Site-specific Transfection of Adherent Cells with siRNA using MEA
  • 08:52Conclusion

Summary

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Automatic Translation

The article details the protocol for site-specific transfection of scrambled sequence of siRNA in an adherent mammalian cell culture using a microelectrode array (MEA).

Tags

SiRNAShRNARNAi PathwayFunctional GenomicsTherapeuticsDelivery TechniquesElectroporationChemical Transfection MethodsCationic LipidsCationic PolymersNanoparticlesHigh-throughput TechnologiesSingle Cell StudiesCell-cell InteractionsGene DeliveryMicroscale Electroporation

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