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JoVE Journal Neuroscience
Analyseren DNA schade in hippocampale neuronen Met behulp van de Comet Assay
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Analyseren DNA schade in hippocampale neuronen Met behulp van de Comet Assay

Article DOI: 10.3791/50049-v • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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De komeet assay is een efficiënte manier voor het opsporen enkel-en dubbel-breuken, met inbegrip van alkali-labiele sites en DNA-DNA/DNA-protein cross-links op het DNA in alle cellen met inbegrip van hippocampale neuronen. De methode maakt gebruik van de differentiële migratie van DNA in een elektrisch veld als gevolg van verschillen in hoeveelheid DNA schade.

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Neuroscience Genetica Cellulaire Biologie Geneeskunde Kankerbiologie Anatomie Fysiologie DNA DNA-schade dubbele breuken enkele streng breuk reparatie neuronen komeet assay celkweek
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