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JoVE Journal Neuroscience
Il saggio danneggiare il DNA in neuroni ippocampali Utilizzo del Comet Assay
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Il saggio danneggiare il DNA in neuroni ippocampali Utilizzo del Comet Assay

Article DOI: 10.3791/50049 • 10:00 min
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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December 19th, 2012

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Il test della cometa è un modo efficiente di rilevare rotture a singolo e doppio filamento, compresi alcali-labili siti e DNA-DNA/DNA-protein legami incrociati sul DNA in tutte le cellule, compresi i neuroni dell'ippocampo. Il metodo sfrutta la migrazione differenziale di DNA in un campo elettrico dovuto a differenze nelle quantità di danno al DNA.

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