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Journal / Bioengineering / Procedure for Decellularization of Porcine Heart…
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JoVE Journal Bioengineering
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
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Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Article DOI: 10.3791/50059-v • 11:30 min • December 6th, 2012
December 6th, 2012 •
1,2, 1,3, 1,2,3,4
1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering, University of Pittsburgh, 3Department of Cardiothoracic Surgery, Children's Hospital of Pittsburgh of UPMC, 4Department of Surgery, University of Pittsburgh

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Summary
Automatic Translation

A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.

Tags

Decellularization Porcine Heart Retrograde Coronary Perfusion Whole Organ Decellularization Tissue Engineering Extracellular Matrix Scaffolds Native Architecture Organ Systems Vascular Network Solutions Of Detergents Acids Enzymatic Treatments Diffusion Distance Decellularization Agents Intact Porcine Heart Coronary Retrograde Perfusion Cardiac Extracellular Matrix (c-ECM) Scaffold Enzymes Detergents Acids Hypertonic Rinses Hypotonic Rinses
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