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JoVE Journal
Bioengineering

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Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
 

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Article DOI: 10.3791/50059
December 6th, 2012

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Summary December 6th, 2012

A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.

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