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Biology

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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
 

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Article DOI: 10.3791/50066
December 17th, 2012

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Summary December 17th, 2012

Here we describe a method to visualize endoplasmic reticulum-associated mRNAs in mammalian tissue culture cells. This technique involves the selective permeabilization of the plasma membrane with digitonin to remove cytoplasmic contents followed by fluorescent in situ hybridization to detect either bulk poly(A) mRNA or specific transcripts.

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