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JoVE Journal
Immunology and Infection

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Real-time Live Imaging of T-cell Signaling Complex Formation
 

Real-time Live Imaging of T-cell Signaling Complex Formation

Article DOI: 10.3791/50076
June 23rd, 2013

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Summary June 23rd, 2013

We describe a live-cell imaging method that provides insight into protein dynamics during the T-cell activation process. We demonstrate the combined usage of the T-cell spreading assay, confocal microscopy and imaging analysis to yield quantitative results to follow signaling complex formation throughout T-cell activation.

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