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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Live beeldvorming van T-cel signaleringscomplex Vorming
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Real-time Live beeldvorming van T-cel signaleringscomplex Vorming

Article DOI: 10.3791/50076-v • 10:31 min • June 23rd, 2013
June 23rd, 2013 •
*1, *1, 1
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University
* These authors contributed equally

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We beschrijven een live-cell imaging methode die inzicht geeft in eiwitdynamica tijdens de T-cel activatie proces. We tonen de totale gebruiksduur van de T-cel verspreiding assay, confocale microscopie en beeldanalyse te geven kwantitatieve resultaten volgen signalering complexvorming in T-cel activatie.

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Immunologie Cellular Biology Molecular Biology Medicine T-celactivering Live-cell imaging signaaltransductie confocale microscopie Signalering complex co-lokalisatie analyse fluorescentie celbiologie T-cel cel imaging
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