Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
 

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Article DOI: 10.3791/50086
November 13th, 2012

Chapters

Summary November 13th, 2012

A rapid way to screen for melanoma modifiers using a zebrafish autochthonous tumor model is presented. It takes advantage of the miniCoopR vector which allows for expression of candidate melanoma genes in melanocytes. A method to obtain melanoma-free survival curves, an invasion assay, a protocol for antibody staining of scale melanocytes and a melanoma transplantation assay are described.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter