19,029 Views
•
15:33 min
•
August 04, 2013
DOI:
Summary
Automatically generated
A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.
Related Videos
08:46
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Related Videos
11901 Views
10:22
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
Related Videos
11153 Views
11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Related Videos
22501 Views
08:05
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Related Videos
8109 Views
09:18
Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
Related Videos
6850 Views
08:21
Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
Related Videos
5814 Views
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
Related Videos
17671 Views
12:26
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
Related Videos
13831 Views
09:48
Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
Related Videos
25883 Views
07:34
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
Related Videos
7402 Views
Read Article
Cite this Article
Rodríguez-Casuriaga, R., Folle, G. A., Santiñaque, F., López-Carro, B., Geisinger, A. Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions. J. Vis. Exp. (78), e50102, doi:10.3791/50102 (2013).
Copy