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Flexural Rigidity Measurements of Biopolymers Using Gliding Assays
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Bioengineering
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JoVE Journal Bioengineering
Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

DOI:
10.3791/50117-v

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07:55 min

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November 09, 2012

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, ,
1Department of Physics,Lawrence University

Chapters

  • 00:05Title
  • 01:01Microtubule Gliding Assay
  • 05:35Fluorescence Imaging and Data Collection
  • 06:48Results: Evaluation of Microtubule Trajectory
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

A method to measure the persistence length or flexural rigidity of biopolymers is described. The method uses a kinesin-driven microtubule gliding assay to experimentally determine the persistence length of individual microtubules and is adaptable to actin-based gliding assays.

Tags

Keywords: MicrotubulesCytoskeletal PolymersCell DivisionCell MechanicsIntracellular TransportPersistence LengthGliding AssaysKinesin-drivenFluorescent LabelingSingle Particle TrackingAutomated TrackingActin FilamentsBiopolymersFlexural Rigidity

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