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Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
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Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

DOI:
10.3791/50151-v

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08:59 min

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August 18, 2013

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, ,
1Department of Anatomy and Cell Biology and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine,Technion-Israel Institute of Technology

Chapters

  • 00:05Title
  • 01:38Preparing Coverslips for Embryo Collection
  • 02:32Embryo Collection and Preparation
  • 05:38Embryo Injection
  • 06:40Imaging the Injected Embryos
  • 07:28Annexin V Injection Results
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe an effective method for studying dynamics of apoptotic cell clearance in vivo. This method employs live Drosophila embryos as a powerful model for monitoring phagocytosis of apoptotic cells using specific labeling of apoptotic cells and phagocytes.

Tags

Keywords: Apoptotic Cell ClearancePhagocytosisDrosophila EmbryogenesisLive ImagingMacrophagesGliaCentral Nervous SystemCell Death

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