Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

Marsha L Quick; Joseph D Done; Praveen Thumbikat

doi:10.3791/50158

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

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06:46 min

January 16, 2013

DOI: 10.3791/50158-v

Marsha L Quick¹, Joseph D Done¹, Praveen Thumbikat¹

¹Department of Urology,Northwestern University Feinberg School of Medicine

Infection of the prostate may be a contributing factor in mediating pelvic pain in chronic prostatitis. We describe the procedure for preparation of standardized bacterial inoculum, instillation of bacteria into the urethra of male mice and methodology for measuring tactile allodynia in mice over time.

The overall aim of this procedure is to measure tactile allodynia in a mouse resulting from a bacterial infection of the prostate. This is accomplished by first preparing a bacterial suspension for installation into a male mouse that is placed under anesthesia. The second step is to gently extrude the penis of the mouse, followed by intraurethral catheterization.

Next bacteria are instilled and initiate an ascending infection of the mouse prostate. The final step is measurement of tactile allodynia following infection. Ultimately, increased response frequency to vray fibers applied to the pelvic area is used to show enhanced tactile elo DIA of the pelvic region in response to bacterial prostatitis.

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